Antihaemolytic activity of thirty herbal extracts in mouse red blood cells

  • Masoumeh Khalili Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari
  • Mohammad Ali Ebrahimzadeh School of Pharmacy, Mazandaran University of Medical Sciences, Sari
  • Yaghoub Safdari School of Pharmacy, Mazandaran University of Medical Sciences, Sari
Keywords: Galium verum, hydrogen peroxide, percolation, phenols, Scutellaria tournefortii, Soxhlet, ultrasound-assisted extraction

Abstract

Reactive oxygen species (ROS) can lead to haemolysis and eventually to diseases such as thalassemia and sickle cell anaemia. Their action can be counteracted by the antihaemolytic activity of therapeutic agents. The aim of our study was to identify plants that most efficiently counteract ROS-caused haemolysis. From ten plants known for their antioxidant activity (Orobanche orientalis G. Beck, Cucumis melo L, Albizzia julibrissin Durazz, Galium verum L, Scutellaria tournefortii Benth, Crocus caspius Fischer & Meyer, Sambucus ebulus L, Danae racemosa L, Rubus fruticsos L, and Artemisia absinthium L.) we prepared 30 extracts using three extraction methods (percolation, Soxhlet, and ultrasound-assisted extraction) to see whether the extraction method affects antihaemolytic efficiency, and one extraction method (polyphenol extraction) to see how much of this action is phenol-related. Extract antihaemolytic activity was determined in mice red blood cells and compared to that of vitamin C as a known antioxidant. Nine of our extracts were more potent than vitamin C, of which G. verum (aerial parts/percolation) and S. tournefortii (aerial parts/polyphenol) extracts were the most potent, with an IC50 of 1.32 and 2.08 µg mL-1, respectively. Haemolysis inhibition depended on extract concentration and the method of extraction. These plants could provide accessible sources of natural antioxidants to the pharmaceutical industry.
Published
2014-11-18
How to Cite
1.
Khalili M, Ebrahimzadeh MA, Safdari Y. Antihaemolytic activity of thirty herbal extracts in mouse red blood cells. Arh Hig Rada Toksikol [Internet]. 2014Nov.18 [cited 2022Oct.1];65(4). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/180
Section
Original article