Protective effects of quercetin and vitamin C against nicotine-induced toxicity in the blood of Wistar rats

  • Milica G Paunovic Faculty of Science, University of Kragujevac, Kragujevac, Serbia
  • Branka I Ognjanovic Faculty of Science, University of Kragujevac, Kragujevac, Serbia
  • Milos M Matic Faculty of Science, University of Kragujevac, Kragujevac, Serbia
  • Andras S Stajn Faculty of Science, University of Kragujevac, Kragujevac, Serbia
  • Zorica S Saicic Department of Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia
Keywords: antioxidant defence, erythrocytes, lipid profile, oxidative damage, reactive oxygen species, tobacco

Abstract

Nicotine is a potential inducer of oxidative stress, through which it can damage numerous biological molecules. The aim of our study was to investigate the prooxidative effects of nicotine and protective (additive or synergistic) effects of quercetin and vitamin C in the blood of experimental animals, to determine whether the combination of these antioxidants might be beneficial for clinical purposes. Wistar albino rats were receiving intraperitoneal nicotine injection (0.75 mg kg-1 per day) or saline (control group) or nicotine plus quercetin (40 mg kg-1 per day) and vitamin C (100 mg kg-1 per day) for three consecutive days. On day 4, we determined their blood lipid profile, liver enzymes, oxidative stress parameters, and antioxidative system parameters. Compared to untreated control, nicotine significantly increased total cholesterol, LDL-cholesterol, triglycerides, liver enzymes (alanine transaminase, aspartate transaminase, and lactate dehydrogenase) and oxidative stress parameters (superoxide anion, hydrogen peroxide, and lipid peroxide) and decreased HDL-cholesterol, glutathione, and superoxide dismutase/catalase activity. Quercetin + vitamin C reversed these values significantly compared to the nicotine alone group. Our results confirm that nicotine has significant prooxidative effects that may disrupt the redox balance and show that the quercetin + vitamin C combination supports antioxidant defence mechanisms with strong haematoprotective activity against nicotine-induced toxicity. In practical terms, this means that a diet rich in vitamin C and quercetin could prevent nicotine-induced toxicity and could also be useful in the supportive care of people exposed to nicotine.

Author Biographies

Milica G Paunovic, Faculty of Science, University of Kragujevac, Kragujevac, Serbia
PhD student
Branka I Ognjanovic, Faculty of Science, University of Kragujevac, Kragujevac, Serbia
Prof.
Milos M Matic, Faculty of Science, University of Kragujevac, Kragujevac, Serbia
PhD student
Andras S Stajn, Faculty of Science, University of Kragujevac, Kragujevac, Serbia
Prof.
Zorica S Saicic, Department of Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia
Prof.
Published
2016-12-15
How to Cite
1.
Paunovic M, Ognjanovic B, Matic M, Stajn A, Saicic Z. Protective effects of quercetin and vitamin C against nicotine-induced toxicity in the blood of Wistar rats. AIHT [Internet]. 15Dec.2016 [cited 19Jul.2019];67(4). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/539
Section
Original article