Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation

  • Jelena Filipović Tričković University of Belgrade Vinča Institute of Nuclear Sciences – National Institute of thе Republic of Serbia, Belgrade, Serbia
  • Ana Valenta Šobot University of Belgrade Vinča Institute of Nuclear Sciences – National Institute of thе Republic of Serbia, Belgrade, Serbia
  • Ivana Joksić Clinic for Gynaecology and Obstetrics Narodni Front, Belgrade, Serbia
  • Gordana Aleksandar Joksić Vinča Institute of Nuclear Science
Keywords: 8-oxo-dG, chromosome aberrations, lipid peroxidation, telomere fragility, telomere length, X-ray

Abstract

Ionising radiation damages DNA directly and indirectly through increased production of reactive oxygen species. Although telomeres have been reported as indicators of radiosensitivity, their maintenance in response to occupational exposure to low radiation doses is still a matter of debate. In this work we aimed to investigate telomere length and structure in hospital workers occupationally exposed to X-rays and to relate these findings to oxidation of biomolecules and chromosome aberrations. Blood samples of exposed participants and matching controls were taken during periodical check-ups. Chromosome aberrations and telomere length and structure were analysed in peripheral blood lymphocytes using Q-FISH, whereas oxidative stress parameters [pro/antioxidant balance (PAB), lipid peroxidation, and 8-oxo-dG] were measured in plasma samples. Based on the CA findings we divided the exposed group into two subgroups, of which one had chromosome aberrations in the first division metaphases and the other did not. There was no significant difference in telomere length between any of the groups. However, both subgroups showed significantly higher rate of fragile telomeres and higher lipid peroxidation product and 8-oxo-dG levels than controls. The rate of fragile telomeres significantly correlated with plasma levels of 8-oxo-dG, which suggests that continuous exposure to low radiation doses induces oxidative base damage of guanine resulting in telomere fragility.

Published
2022-03-23
How to Cite
1.
Filipović Tričković J, Valenta Šobot A, Joksić I, Joksić GA. Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation. Arh Hig Rada Toksikol [Internet]. 2022Mar.23 [cited 2022Jun.30];73(1). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/1458
Section
Original article