Naringenin ameliorates pathological changes in liver and kidney of diabetic mice: a preliminary study

  • Damir Sirovina Department of Biology, Division of Zoology, Faculty of Science, University of Zagreb, Zagreb
  • Nada Oršolić Department of Biology, Division of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb
  • Marijana Zovko Končić Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb
  • Gordana Gregorović Department of Biology, Division of Zoology, Faculty of Science, University of Zagreb, Zagreb
Keywords: diabetes, diabetic kidney, histopathology, lipid peroxidation, liver disease, naringenin

Abstract

The effect of naringenin, a flavonoid found in grapefruit, orange, and tomato, on lipid peroxidation and histopathological changes in the liver and kidneys of alloxan-induced diabetic mice were investigated. Two days after alloxan injection (75 mg kg-1,i.v.), naringenin ethanolic solution (0.5 % v/v) was given to mice intraperitoneally (50 mg kg-1 per day) for seven days. Naringenin's impact on lipid peroxidation was measured by the 2-thiobarbituric acid test and histopathological changes were examined under a light microscope. Naringenin administration resulted in a significant decrease of lipid peroxidation level in liver and kidney tissue, as well as in a decreased number of vacuolated liver cells and degree of vacuolisation. Indications of tissue repair in kidney suggested that amelioration of diabetes-induced renal damage could be achieved over a longer period of time. Findings suggest that naringenin could be considered a dietary supplement in the prevention or treatment of diabetic complications and other diseases connected wih oxidative stress, and gives a hope that it could show similar effects in the treatment of diabetes in humans.

Published
2016-03-29
How to Cite
1.
Sirovina D, Oršolić N, Zovko Končić M, Gregorović G. Naringenin ameliorates pathological changes in liver and kidney of diabetic mice: a preliminary study. Arh Hig Rada Toksikol [Internet]. 2016Mar.29 [cited 2021Oct.23];67(1). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/432
Section
Original article