Renal changes and apoptosis caused by subacute exposure to Aroclor 1254 in selenium-deficient and selenium-supplemented rats

  • Naciye Dilara Zeybek Department of Histology and Embryology, Hacettepe University, Faculty of Medicine, Ankara, Turkey
  • Unzile Sur Department of Toxicology, Hacettepe University, Faculty of Pharmacy, Ankara, Turkey
  • Ofcan Oflaz Department of Biology, Hacettepe University, Faculty of Science, Ankara, Turkey
  • Pinar Erkekoglu Department of Toxicology, Hacettepe University, Faculty of Pharmacy, Ankara, Turkey
  • Aylin Balci Department of Toxicology, Hacettepe University, Faculty of Pharmacy, Ankara, Turkey
  • Gizem Ozkemahli Department of Toxicology, Hacettepe University, Faculty of Pharmacy, Ankara, Turkey
  • Ali Asci Department of Toxicology, Atatürk University, Faculty of Pharmacy, Erzurum, Turkey
  • Murat Kizilgun Dışkapı Pediatric Health and Disease Hematology, Oncology Training and Research Hospital, Ankara, Turkey
  • Oguz Han Edebal Dışkapı Pediatric Health and Disease Hematology, Oncology Training and Research Hospital, Ankara, Turkey
  • Belma Kocer-Gumusel
Keywords: antioxidant enzymes, electron microscopy, histopathology, kidney, oxidative stress, polychlorinated biphenyls, Sprague-Dawley rats, TUNEL assay, ultrastructural changes

Abstract

Aroclor 1254 (A1254), a mixture of polychlorinated biphenyls, exerts hepatic, renal, and reproductive toxicity in rodents. This study aimed to determine a protective role of selenium on histopathological changes, oxidative stress, and apoptosis caused by A1254 in rat kidney. It included a control group, which received regular diet containing 0.15 mg/kg Se (C), a Se-supplemented group (SeS) receiving 1 mg/kg Se, a Se-deficient group (SeD) receiving Se-deficient diet of ≤0.05 mg/kg Se, an A1254-treated group (A) receiving 10 mg/kg of Aroclor 1254 and regular diet, an A1254-treated group receiving Se-supplementation (ASeS), and an A1254-treated group receiving Se-deficient diet (ASeD). Treatments lasted 15 days. After 24 h of the last dose of A1254, the animals were decapitated under anaesthesia and their renal antioxidant enzyme activities, lipid peroxidation (LP), glutathione, protein oxidation, and total antioxidant capacity levels measured. Histopathological changes were evaluated by light and electron microscopy. Apoptosis was detected with the TUNEL assay. Kidney weights, CAT activities, and GSH levels decreased significantly in all A1254-treated groups. Renal atrophic changes and higher apoptotic cell counts were observed in the A and ASeD groups. Both groups also showed a significant drop in GPx1 activities (A – 34.92 % and ASeD – 86.46 %) and rise in LP (A – 30.45 % and ASeD – 20.44 %) vs control. In contrast, LP levels and apoptotic cell counts were significantly lower in the ASeS group vs the A group. Histopathological changes and renal apoptosis were particularly visible in the ASeD group. Our findings suggest that selenium supplementation provides partial protection against renal toxicity of Aroclor 1254.

Published
2020-06-15
How to Cite
1.
Zeybek ND, Sur U, Oflaz O, Erkekoglu P, Balci A, Ozkemahli G, Asci A, Kizilgun M, Edebal OH, Kocer-GumuselB. Renal changes and apoptosis caused by subacute exposure to Aroclor 1254 in selenium-deficient and selenium-supplemented rats. Arh Hig Rada Toksikol [Internet]. 2020Jun.15 [cited 2020Dec.3];71(2). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/1208
Section
Original article

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