Aqueous sage leave extract attenuates inflammation and oxidant-induced genotoxicity in human peripheral blood mononuclear cells

  • Ana Valenta Šobot University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
  • Marijana Janić University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
  • Iva Popović University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
  • Tamara Lazarević-Pašti University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
  • Tatjana Momić University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
  • Aleksandar Krstić University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Physical Chemistry, Belgrade, Serbia
  • Jelena Filipović Tričković Institute of Nuclear Sciences – National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia
Keywords: AChE inhibition, antigenotoxic effects, anti-inflammatory effects, antioxidant, Salvia officinalis L.

Abstract

Traditional medicine has used sage (Salvia officinalis L.) preparations for centuries to prevent and treat various inflammatory and oxidative stress-induced conditions. The aim of this in vitro study was to determine the bioactive properties of a sage leave extract obtained with environmentally friendly aqueous extraction and lyophilisation in primary human peripheral blood cells. To that end we measured the total phenolic and flavonoid content (TPC and TFC, respectively) with gas chromatography-mass spectrometry (GC-MS). Non-cytotoxic concentrations determined with the trypan blue assay were used to assess the antioxidant (DPPH, ABTS, and PAB assay), antigenotoxic (CBMN assay), immunomodulatory (IL-1β and TNF-α), and neuroprotective effects (AChE inhibition). The extract contained high TPC (162 mg GAE/g of dry extract) and TFC (39.47 mg QE/g of dry extract) concentrations, while β-thujone content was unexpectedly low (below 0.9 %). Strong radical-scavenging activity combined with glutathione reductase activation led to a decrease in basal and H2O2-induced oxidative stress and DNA damage. A decrease in TNF-α and increase in IL-1β levels suggest complex immunomodulatory response that could contribute to antioxidant and, together with mild AChE inhibition, neuroprotective effects. Overall, this study has demonstrated that aqueous sage leave extract reduces the levels of thujone, 1,8-cineole, pinene, and terpene ketones that could be toxic in high concentrations, while maintaining high concentrations of biologically active protective compounds which have a potential to prevent and/or treat inflammatory and oxidative stress-related conditions.

Published
2024-05-10
How to Cite
1.
Valenta Šobot A, Janić M, Popović I, Lazarević-Pašti T, Momić T, Krstić A, Filipović Tričković J. Aqueous sage leave extract attenuates inflammation and oxidant-induced genotoxicity in human peripheral blood mononuclear cells. Arh Hig Rada Toksikol [Internet]. 2024May10 [cited 2024Jul.14];75(2). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/1700
Section
Original article

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