ABCB1, CYP2B6, and CYP3A4 genetic polymorphisms do not affect methadone maintenance treatment in HCV-positive patients

  • Davorka Sutlović Sveučilište u Splitu; Sveučilišni odjel zdravstvenih studija
  • Zeljko Kljucevic Department of mental health, prevention and outpatient addiction treatment, Public Health Institute of Split - Dalmatian County, 21 000 Split, Croatia
  • Sendi Kuret Department of Pathology and Forensic Medicine, University Hospital Centre Split, 21 000 Split, Croatia
Keywords: EDDP, genotyping, hepatitis C, liver damage, SNP

Abstract

The aim of this study was to determine the influence of ABCB1, CYP2B6, and CYP3A4 genetic polymorphisms on methadone metabolism in patients with hepatitis C virus (HCV) undergoing methadone maintenance treatment (MMT). The study included 35 participants undergoing MMT, who were divided in three groups: HCV-positive (N=12), HCV-negative (N=16), and HCV clinical remission (CR) (N=7). The concentrations of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) were determined with gas chromatography-mass spectrometry. The patients were genotyped for ABCB1 rs1045642, CYP2B6 rs3745274, CYP3A4 rs2242480, and CYP3A4 rs2740574 polymorphisms. Differences between single nucleotide polymorphism (SNP) genotypes and methadone-to-EDDP ratio were analysed with one-way ANOVA, which showed no significant difference between the genes (p=0.3772 for ABCB1 rs1045642, p=0.6909 for CYP2B6 rs3745274, and p=0.6533 for CYP3A4 rs2242480). None of the four analysed SNP genotypes correlated with methadone-to-EDDP concentration ratio. A major influence on it in hepatitis C-positive patients turned out to be the stage of liver damage.

Published
2020-11-11
How to Cite
1.
Sutlović D, Kljucevic Z, Kuret S. ABCB1, CYP2B6, and CYP3A4 genetic polymorphisms do not affect methadone maintenance treatment in HCV-positive patients. Arh Hig Rada Toksikol [Internet]. 2020Nov.11 [cited 2021Jul.31];71(4). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/1227
Section
Original article