In vivo changes in carbonic anhydrase activity and histopathology of gill and liver tissues after acute exposure to chlorpyrifos in rainbow trout

  • Ahmet Topal Department of Basic Sciences, Faculty of Fisheries, Ataturk University, TR-25240 Erzurum
  • Muhammed Atamanalp Department of Aquaculture, Faculty of Fisheries, Ataturk University, TR-25240 Erzurum
  • Ertan Oruç Department of Pathology, Faculty of Veterinary, Ataturk University, TR-25240 Erzurum
  • Şükrü Beydemir Department of Chemistry, Faculty of Science, Ataturk University, TR-25240 Erzurum
  • Alparslan Işık Department of Basic Sciences, Faculty of Fisheries, Ataturk University, TR-25240 Erzurum
  • Yeliz Demir Department of Chemistry, Faculty of Science, Ataturk University, TR-25240 Erzurum
Keywords: acute toxicity, fish, histology, light microscopy, organophosphate pesticides

Abstract

Chlorpyrifos is an organophosphate pesticide widely used in agriculture and aquaculture. This study investigated its effect on carbonic anhydrase (CA) enzyme activity and histopathology of rainbow trout gill and liver. The fish were exposed to 2.25 (25 % of 96 h LC50), 4.5 (50 % of 96 h LC50), and 6.75 µg L-1 (75 % of 96 h LC50) of chlorpyrifos for 24, 48, 72, and 96 h. CA activity was measured in liver and gills and histopathological changes were examined by light microscopy. The most common liver changes at most of the chlorpyrifos concentrations were hyperaemia and degenerative changes. Gill tissues were characterised by lamellar hyperaemia, lamellar oedemas, clumping, cellular degeneration, hyperplasia, and lamellar atrophy. CA enzyme activity in the gills decreased at all concentrations at 48, 72, and 96 h after exposure to chlorpyrifos (p<0.05). Similarly, there was a time-dependent decrease in CA activity at all of the concentrations in liver tissues (p<0.05). The present study indicated that chlorpyrifos inhibits CA enzyme activity and causes histopathological damage in gill and liver tissues.
Published
2014-11-17
How to Cite
1.
Topal A, Atamanalp M, Oruç E, Beydemir Şükrü, Işık A, Demir Y. In vivo changes in carbonic anhydrase activity and histopathology of gill and liver tissues after acute exposure to chlorpyrifos in rainbow trout. Arh Hig Rada Toksikol [Internet]. 2014Nov.17 [cited 2022Oct.1];65(4). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/228
Section
Original article