L-glutamic acid-g-poly hydroxyethyl methacrylate nanoparticles: acute and sub-acute toxicity and biodistribution potential in mice

  • Buket Bakan Atatürk University, Faculty of Science, Department of Molecular Biology and Genetics, 25240, Erzurum, Turkey
  • Fatih Oltulu Ege University, Faculty of Medicine, Department of Histology and Embryology, 35100, Izmir Turkey
  • Yeliz Yıldırım Ege University, Faculty of Science, Department of Chemistry, 35100, Izmir, Turkey
  • Altug Yavasoglu Ege University, Faculty of Medicine, Department of Histology and Embryology, 35100, Izmir Turkey
  • Sinan Akgol Ege University, Faculty of Science, Department of Biochemistry, 35100, Izmir, Turkey
  • Nefise Ulku Karabay Yavasoglu Ege University, Faculty of Science, Department of Biology, 35100, Izmir Turkey
Keywords: biocompatibility, blood biochemistry, genotoxicity, histology, in vivo toxicity, micronucleus test, polymers

Abstract

The aim of this safety study in mice was to determine in vivo toxicity and biodistribution potential of a single and multiple doses of L-glutamic acid-g-p(HEMA) polymeric nanoparticles as a drug delivery system. The single dose did not cause any lethal effect, and its acute oral LD50 was >2.000 mg/kg body weight (bw). Multiple doses (25, 50, or 100 mg/kg bw) given over 28 days resulted in no significant differences in body and relative organ weights compared to control. These results are supported by biochemical and histological findings. Moreover, nanoparticle exposure did not result in statistically significant differences in micronucleus counts in bone marrow cells compared to control. Nanoparticle distribution was time-dependent, and they reached the organs and even bone marrow by hour 6, as established by ex vivo imaging with the IVIS spectrum. In conclusion, L-glutamic acid-g-p(HEMA) polymeric nanoparticles appear biocompatible and have a potential use as a drug delivery system.

Published
2023-09-13
How to Cite
1.
Bakan B, Oltulu F, Yıldırım Y, Yavasoglu A, Akgol S, Karabay Yavasoglu NU. L-glutamic acid-g-poly hydroxyethyl methacrylate nanoparticles: acute and sub-acute toxicity and biodistribution potential in mice. Arh Hig Rada Toksikol [Internet]. 2023Sep.13 [cited 2024Jun.22];74(3). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/1624
Section
Original article