Genotoxic effects of diazinon on human peripheral blood lymphocytes

  • Fulya Dilek Gökalp Muranlı Trakya University
  • Martin Kanev Trakya University
  • Kezban Özdemir Trakya University
Keywords: comet assay, FISH, genotoxicity, micronucleus, organophosphate pesticides

Abstract

The aim of this study was to evaluate the genetic damage in human peripheral blood lymphocytes following 24 and 48-hour exposure to a commercial diazinon formulation Basudin 60EM® at concentrations between 0.01 and 40 µg mL-1. For this purpose we used the micronucleus (MN), fluorescence in situ hybridization (FISH), and alkaline single cell gel electrophoresis (comet) assay. Diazinon significantly increased the frequency of micronucleated cells compared to control. Forty-eight-hour exposure increased this frequency even at lower concentrations (0.01-10 µg mL-1). The FISH results revealed aneugenic effects at 10 µg mL-1. The comet assay also confirmed DNA damage at concentrations between 10 and 40 µg mL-1. Our findings have confirmed the genotoxic potential of diazinon and its cytotoxic effect on human lymphocytes. The increased DNA damage in our study raises concern about the current assessment of the health risk posed by this pesticide and calls for a high level of caution in agricultural and household use.

Author Biographies

Fulya Dilek Gökalp Muranlı, Trakya University
Department of Biology
Martin Kanev, Trakya University
Department of Biology
Kezban Özdemir, Trakya University
Department of Biology
Published
2015-06-08
How to Cite
1.
Gökalp Muranlı FD, Kanev M, Özdemir K. Genotoxic effects of diazinon on human peripheral blood lymphocytes. Arh Hig Rada Toksikol [Internet]. 2015Jun.8 [cited 2024Dec.1];66(2). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/278
Section
Original article