Mutagenicity and DNA Damage of Bisphenol A and Its Structural Analogues in HepG2 Cells

Authors

  • Anja Fic Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
  • Bojana Žegura Department for Genetic Toxicology and Cancer Biology, Ljubljana, Slovenia
  • Marija Sollner Dolenc Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
  • Metka Filipič Department for Genetic Toxicology and Cancer Biology, Ljubljana, Slovenia
  • Lucija Peterlin Mašič Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia

DOI:

https://doi.org/10.2478/10004-1254-64-2013-2319

Keywords:

Ames test, bisphenols, comet assay, genotoxicity, mutagenicity

Abstract

Environmental oestrogen bisphenol A (BPA) and its analogues are widespread in our living environment. Because their production and use are increasing, exposure of humans to bisphenols is becoming a significant issue. We evaluated the mutagenic and genotoxic potential of eight BPA structural analogues (BPF, BPAF, BPZ, BPS, DMBPA, DMBPS, BP-1, and BP-2) using the Ames and comet assay, respectively. None of the tested bisphenols showed a mutagenic effect in Salmonella typhimurium strains TA98 and TA100 in either the presence or absence of external S9-mediated metabolic activation (Aroclor 1254-induced male rat liver). Potential genotoxicity of bisphenols was determined in the human hepatoma cell line (HepG2) at non-cytotoxic concentrations (0.1 µmol L-1 to 10 µmol L-1) after 4-hour and 24-hour exposure. In the comet assay, BPA and its analogue BPS induced significant DNA damage only after the 24-hour exposure, while analogues DMBPS, BP-1, and BP-2 induced a transient increase in DNA strand breaks observed only after the 4-hour exposure. BPF, BPAF, BPZ, and DMBPA did not induce DNA damage.

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Published

10.06.2013

Issue

Section

Scientific Paper

How to Cite

1.
Fic A, Žegura B, Sollner Dolenc M, Filipič M, Peterlin Mašič L. Mutagenicity and DNA Damage of Bisphenol A and Its Structural Analogues in HepG2 Cells. Arh Hig Rada Toksikol [Internet]. 2013 Jun. 10 [cited 2025 Apr. 2];64(2). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/62

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