Redox and biometal status in Wistar rats after subacute exposure to fluoride and selenium counter-effects

  • Jelena Radovanović University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Radiobiology and Molecular Genetics, Belgrade, Serbia
  • Biljana Antonijević University of Belgrade Faculty of Pharmacy, Department of Toxicology "Akademik Danilo Soldatović", Belgrade, Serbia
  • Katarina Baralić University of Belgrade Faculty of Pharmacy, Department of Toxicology "Akademik Danilo Soldatović", Belgrade, Serbia
  • Marijana Ćurčić University of Belgrade Faculty of Pharmacy, Department of Toxicology "Akademik Danilo Soldatović", Belgrade, Serbia
  • Danijela Đukić-Ćosić University of Belgrade Faculty of Pharmacy, Department of Toxicology "Akademik Danilo Soldatović", Belgrade, Serbia
  • Zorica Bulat University of Belgrade Faculty of Pharmacy, Department of Toxicology "Akademik Danilo Soldatović", Belgrade, Serbia
  • Dragana Javorac University of Belgrade Faculty of Pharmacy, Department of Toxicology "Akademik Danilo Soldatović", Belgrade, Serbia
  • Aleksandra Buha Đorđević University of Belgrade Faculty of Pharmacy, Department of Toxicology "Akademik Danilo Soldatović", Belgrade, Serbia
  • Jelena Kotur-Stevuljević University of Belgrade Faculty of Pharmacy, Department of Biochemistry, Belgrade, Serbia
  • Emina Sudar-Milovanović University of Belgrade, Vinča Institute of Nuclear Sciences, Department of Radiobiology and Molecular Genetics, Belgrade, Serbia
  • Evica Antonijević Miljaković University of Belgrade Faculty of Pharmacy, Department of Toxicology "Akademik Danilo Soldatović", Belgrade, Serbia
  • Miloš Beloica University of Belgrade School of Dental Medicine, Clinic for Paediatric and Preventive Dentistry, Belgrade, Serbia
  • Zoran Mandinić University of Belgrade School of Dental Medicine, Clinic for Paediatric and Preventive Dentistry, Belgrade, Serbia
DOI: https://doi.org/10.2478/aiht-2022-73-3650
Keywords: Cu, Fe, MDA, NaF, O2-, oxidative stress, Se, SOD1, sodium fluoride, subacute toxicity, Zn

Abstract

This study aimed to investigate the effect of 150 mg/L sodium fluoride (NaF) on redox status parameters and essential metals [copper (Cu), iron (Fe), and zinc (Zn)] in the blood, liver, kidney, brain, and spleen of Wistar rats and to determine the protective potential of selenium (Se) against fluoride (F-) toxicity. Male Wistar rats were randomly distributed in groups of five (n=5) receiving tap water (control) or water with NaF 150 mg/L, NaF 150 mg/L + Se 1.5 mg/L, and Se 1.5 mg/L solutions ad libitum for 28 days. Fluorides caused an imbalance in the redox and biometal (Cu, Fe, and Zn) status, leading to high superoxide anion (O2.-) and malondialdehyde (MDA) levels in the blood and brain and a drop in superoxide dismutase (SOD1) activity in the liver and its increase in the brain and kidneys. Se given with NaF improved MDA, SOD1, and O2.- in the blood, brain, and kidneys, while alone it decreased SH group levels in the liver and kidney. Biometals both reduced and increased F- toxicity. Further research is needed before Se should be considered as a promising strategy for mitigating F- toxicity.

Published
2022-09-15
How to Cite
1.
Radovanović J, Antonijević B, Baralić K, Ćurčić M, Đukić-Ćosić D, Bulat Z, Javorac D, Buha Đorđević A, Kotur-Stevuljević J, Sudar-Milovanović E, Antonijević Miljaković E, Beloica M, Mandinić Z. Redox and biometal status in Wistar rats after subacute exposure to fluoride and selenium counter-effects. Arh Hig Rada Toksikol [Internet]. 2022Sep.15 [cited 2024Apr.19];73(3). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/1500
Issue
Vol 73 No 3 (2022)
Section
Original article

Copyright (c) 2022 Jelena Radovanović, Biljana Antonijević, Katarina Baralić, Marijana Ćurčić, Danijela Đukić-Ćosić, Zorica Bulat, Dragana Javorac, Aleksandra Buha Đorđević, Jelena Kotur-Stevuljević, Emina Sudar-Milovanović, Evica Antonijević Miljaković, Miloš Beloica, Zoran Mandinić

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