The influence of shear stress on the adhesion capacity of Legionella pneumophila

  • Martina Oder University of Ljubljana, Faculty of Health Sciences
  • Rok Fink University of Ljubljana, Faculty of Health Sciences
  • Klemen Bohinc University of Ljubljana, Faculty of Health Sciences
  • Karmen Godič Torkar University of Ljubljana, Faculty of Health Sciences

Abstract

Bacterial adhesion is a complex process influenced by many factors, including hydrodynamic conditions. They affect the transfer of oxygen, nutrients, and bacterial cells in a water supply and cooling systems. The aim of this study was to identify hydrodynamic effects on bacterial adhesion to and detachment from stainless steel surfaces. For this purpose we observed the behaviour of bacterium L. pneumophila in no-flow and laminar and turbulent flow conditions simulated in a fluid flow chamber. The bacterial growth in no-flow and laminar flow conditions was almost identical in the first 24 h, while at 48 and 72 h of incubation, the laminar flow stimulated bacterial growth. In the second part of this study we found that laminar flow accelerated bacterial adhesion in the first 48 h, but after 72 h the amount of bacterial cells exposed to the flow dropped, probably due to detachment. In the third part we found that the turbulent flow detached more bacterial cells than the laminar, which indicates that the strength of shear forces determines the rate of bacterial removal.

Published
2017-05-19
How to Cite
ODER, Martina et al. The influence of shear stress on the adhesion capacity of Legionella pneumophila. Archives of Industrial Hygiene and Toxicology, [S.l.], v. 68, n. 2, may 2017. ISSN 1848-6312. Available at: <https://arhiv.imi.hr/index.php/arhiv/article/view/667>. Date accessed: 22 aug. 2017. doi: https://doi.org/10.1515/aiht-2017-68-2904.
Section
Original article

Keywords

Legionella pneumophila, adhesion, shear stress, flow chamber