Degradation of soil cyanide by single and mixed cultures of Pseudomonas stutzeri and Bacillus subtilis

Abstract

The aim of this investigation was to study whether certain bacteria could be used for cyanide degradation in soil. The bacteria Pseudomonas stutzeri and Bacillus subtilis were selected based on their good growth in a minimal medium containing 0.8 mg mL^-1 potassium cyanide (KCN). In this study we tested their ability to reduce cyanide levels in a medium containing 1.5 mg mL^-1 of KCN. Although both microorganisms reduced cyanide levels, Pseudomonas stutzeri was the more effective test organism. Later on, the selected cultures were grown, diluted and their various cell concentrations were used individually and in combination to test their ability of cyanide degradation in soil samples collected around a cassava processing mill. Bacillus subtilis caused degradation of soil cyanide from 0.218 mg g^-1 soil immediately with an inoculum concentration of 0.1 (OD_600nm) to 0.072 mg g^-1 soil after 10 days with an inoculum concentration of 0.6 (OD_600nm) implying a 66.9 % reduction. Pseudomonas stutzeri cell concentration of 0.1 (OD_600nm) decreased soil cyanide from 0.218 mg g^-1 soil initially to 0.061 mg g^-1 soil after 10 days with an inoculum concentration of 0.6 (OD_600nm)(72 % reduction). The mixed culture of the two bacteria produced the best degradation of soil cyanide from 0.218 mg g^-1 soil sample with a combined inoculum concentration of 0.1 (OD_600nm) initially to 0.025 mg g^-1 soil with a combined inoculum concentration of 0.6 (OD_600nm) after 10 days incubation resulting in an 88.5 % degradation of soil cyanide. The analysed bacteria displayed high cyanide degradation potential and may be useful for efficient decontamination of cyanide contaminated sites.