First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm

  • Ivana Bošnjak Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology
  • Ana Bielen Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology
  • Sanja Babić Division of Materials Chemistry, Ruđer Bošković Institute
  • Lidija Šver Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology
  • Natalija Topić Popović Division of Materials Chemistry, Ruđer Bošković Institute
  • Ivančica Strunjak-Perović Division of Materials Chemistry, Ruđer Bošković Institute
  • Rozelinda Čož-Rakovac Division of Materials Chemistry, Ruđer Bošković Institute
  • Roberta Sauerborn Klobučar Division of Materials Chemistry, Ruđer Bošković Institute
DOI: https://doi.org/10.2478/10004-1254-65-2014-2421
Keywords: ABC transport proteins, cellular detoxification, cyclosporine A, Eisenia fetida, MK571, MXR, orthovanadate, probenecid, soil organism, verapamil

Abstract

Multixenobiotic resistance (MXR) is an important mechanism of cellular efflux mediated by ATP binding cassette (ABC) transporters that bind and actively remove toxic substrates from the cell. This study was the first to identify ABC transporter P-glycoprotein (P-gp/ABCB1) as a representative of the MXR phenotype in earthworm (Eisenia fetida). The identified partial cDNA sequence of ABCB1 overlapped with ABCB1 homologues of other organisms from 58.5 % to 72.5 %. We also studied the effect of five modulators (verapamil, cyclosporine A, MK571, probenecid, and orthovanadate) on the earthworm’s MXR activity by measuring the accumulation of model substrates rhodamine B and rhodamine 123 in whole body tissue of the adult earthworm. MK571, orthovanadate, and verapamil significantly inhibited MXR activity, and rhodamine 123 turned out to better reflect MXR activity in that species than rhodamine B. Our results show that E. fetida can serve well as a test organism for environmental pollutants that inhibit MXR activity. 

Published
2014-03-01
How to Cite
1.
Bošnjak I, Bielen A, Babić S, Šver L, Topić Popović N, Strunjak-Perović I, Čož-Rakovac R, Sauerborn Klobučar R. First evidence of the P-glycoprotein gene expression and multixenobiotic resistance modulation in earthworm. Arh Hig Rada Toksikol [Internet]. 2014Mar.1 [cited 2024Apr.27];65(1). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/163
Issue
Vol 65 No 1 (2014)
Section
Original article