Age and sex differences in genome damage between prepubertal and adult mice after exposure to ionising radiation

  • Ranko Stojković Rudjer Boskovic Institute, Zagreb
  • Aleksandra Fučić Institute for Medical Research and Occupational Health, Zagreb
  • Dušica Ivanković Rudjer Boskovic Institute, Zagreb
  • Zoran Jukić General Hospital “Nova Gradiska”, Nova Gradiska
  • Petra Radulović Clinical Hospital Centre “Sestre Milosrdnice”, Zagreb
  • Josip Grah University Hospital „Zagreb“, Zagreb
  • Nenad Kovačević University Hospital „Zagreb“, Zagreb
  • Lovro Barišić University Hospital „Zagreb“, Zagreb
  • Božo Krušlin Clinical Hospital Centre “Sestre Milosrdnice”, Zagreb
Keywords: gamma radiation, micronucleus, oestrogen, radiosensitivity, testosterone

Abstract

The mechanisms that lead to sex and age differences in biological responses to exposure to ionising radiation and related health risks have still not been investigated to a satisfactory extent. The significance of sex hormones in the aetiology of radiogenic cancer types requires a better understanding of the mechanisms involved, especially during organism development. The aim of this study was to show age and sex differences in genome damage between prepubertal and adult mice after single exposure to gamma radiation. Genome damage was measured 24 h, 48 h, and 72 h after exposure of 3-week and 12-week old BALB/CJ mice to 8 Gy of gamma radiation using an in vivo micronucleus assay. There was a significantly higher genome damage in prepubertal than in adult animals of both sexes for all sampling times. Irradiation caused a higher frequency of micronuclei in males of both age groups. Our study confirms sex differences in the susceptibility to effects of ionising radiation in mice and is the first to show that such a difference occurs already at prepubertal age.
Published
2016-12-15
How to Cite
1.
Stojković R, Fučić A, Ivanković D, Jukić Z, Radulović P, Grah J, Kovačević N, Barišić L, Krušlin B. Age and sex differences in genome damage between prepubertal and adult mice after exposure to ionising radiation. AIHT [Internet]. 15Dec.2016 [cited 19Jul.2019];67(4). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/636
Section
Original article