Antioxidative, cytotoxic, and antibacterial properties of self-assembled glycine-histidine-based dipeptides with or without silver nanoparticles in bio-inspired film

  • Merve Eylul Kiymaci University of Health Sciences, Gülhane Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Ankara, Turkey
  • Hakan Erdoğan University of Health Sciences, Gülhane Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Ankara, Turkey
  • Merve Bacanlı University of Health Sciences, Gülhane Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey
Keywords: Ag, antibacterial surface, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, toxicity

Abstract

Recent years have seen much attention being given to self-assembly of dipeptide-based structures, especially to self-regulation of dipeptide structures with different amino acid sequences. In this study we investigated the effects of varying solvent environments on the self-assembly of glycine-histidine (Gly-His) dipeptide structures. First we determined the morphological properties of Gly-His films formed in different solvent environments with scanning electron microscopy and then structural properties with Fourier-transform infrared (FTIR) spectroscopy. In addition, we studied the effects of Gly-His films on silver nanoparticle (AgNP) formation and the antioxidant and cytotoxic properties of AgNPs obtained in this way. We also, assessed antibacterial activities of Gly-His films against Gram-negative Escherichia coli and Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus. Silver nanoparticle-decorated Gly-His films were not significantly cytotoxic at concentrations below 2 mg/mL but had antibacterial activity. We therefore believe that AgNP-decorated Gly-His films at concentrations below 2 mg/mL can be used safely against bacteria.

Published
2022-06-10
How to Cite
1.
Kiymaci ME, Erdoğan H, Bacanlı M. Antioxidative, cytotoxic, and antibacterial properties of self-assembled glycine-histidine-based dipeptides with or without silver nanoparticles in bio-inspired film. Arh Hig Rada Toksikol [Internet]. 2022Jun.10 [cited 2024Mar.28];73(2). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/1508
Section
Original article