Chemical and biological characteristics of propolis from Apis mellifera caucasica from the Ardahan and Erzurum provinces of Turkey: a comparative study

Keywords: anticarcinogen, antimicrobial, biological activity, mineral, mitomycin C, organic composition

Abstract

The aim of this study was to compare the biological activities of ethanolic propolis extracts of Apis mellifera caucasica obtained from Ardahan and Erzurum provinces of Turkey. Samples were tested for antioxidant, anticytotoxic, anticarcinogenic, antibacterial, and antifungal potentials using different techniques. Propolis samples from the two provinces had different mineral and organic compositions related to their geographical origin. The ferric reducing antioxidant power (FRAP) test showed superiority of Ardahan propolis over the Erzurum. Regardless of origin and the presence of mitomycin C in the culture medium, propolis enhanced human peripheral lymphocyte viability, which depended on the duration and propolis concentration. Antiperoxidative activity on MCF-7 breast cancer cells was concentration-dependent. Erzurum propolis showed the highest anticarcinogenic activity at the concentrations of 62.5 µg/mL and 125 µg/mL, which dropped at higher concentrations. All propolis samples also showed antibacterial activity against the tested human pathogens similar to ampicillin and penicillin controls, except for Pseudomonas aeruginosa. However, they did not exert any antifungal activity against Candida albicans and Yarrowia lipolytica. In conclusion, propolis samples from both provinces showed promising biological activities, but further research should focus on finding the right concentrations for optimal effect and include the cell necrosis pathway to get a better idea of the anticarcinogenic effects.

Author Biographies

Mehmet Arslan, Ardahan University

Faculty of Health Sciences, Department of Health Management

Celal Güven, Niğde Ömer Halisdemir University

Faculty of Medicine, Department of Biophysics

Zehra Tuğba Murathan, Malatya Turgut Özal University

Battalgazi Vocational School

Nurcan Erbil, Ardahan University

Faculty of Health Sciences, Department of Nursing

Deniz Yıldırım, Çukurova University

Faculty of Ceyhan Engineering, Department of Chemical Engineering

Mehmet Büyükleyla, Ardahan University

Ardahan Vocational School of Health Services

Şakire Karadaş, Kahramanmaraş Sütçü İmam University

Faculty of Science and Letters, Department of Biology

Rima Çelik, Kilis 7 Aralık University

Faculty of Science and Letters, Department of Molecular Biology and Genetics

Eyyüp Rencüzoğulları, Adiyaman University

Faculty of Science and Letters, Department of Biology

Published
2021-03-04
How to Cite
1.
Arslan M, Sevgiler Y, Güven C, Murathan ZT, Erbil N, Yıldırım D, Büyükleyla M, Karadaş Şakire, Çelik R, Rencüzoğulları E. Chemical and biological characteristics of propolis from Apis mellifera caucasica from the Ardahan and Erzurum provinces of Turkey: a comparative study. Arh Hig Rada Toksikol [Internet]. 2021Mar.4 [cited 2024Mar.29];72(1). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/1348
Section
Original article