Toxicopathological changes induced by combined exposure to noise and toluene in New Zealand White rabbits

  • Amirreza Abouee-Mehrizi Tabriz University of Medical Sciences Faculty of Health, Department of Occupational Health Engineering, Tabriz, Iran
  • Yahya Rasoulzadeh Tabriz University of Medical Sciences Faculty of Health, Department of Occupational Health Engineering, Tabriz, Iran
  • Tohid Kazemi Tabriz University of Medical Sciences Immunology Research Centre, Tabriz, Iran
  • Ahmad Mehdipour Tabriz University of Medical Sciences Faculty of Advanced Medical Sciences, Department of Tissue Engineering, Tabriz, Iran
  • Mehran Mesgari-Abbasi Tabriz University of Medical Sciences, Drug Applied Research Centre, Tabriz, Iran
Keywords: body weight, environmental exposure, heart, IL-1β, industrial toxicology, apoptosis, lung, spleen, stomach, TNF-α

Abstract

Noise and toluene can have significant adverse effects on different systems in the human body, but little is known about their combination. The aim of this study was to see how their combined action reflects on serum levels of inflammatory cytokines tumour necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β), body weight, and pathological changes in the heart, lung, stomach, and spleen tissues. To do that we exposed New Zealand rabbits to 1000 mg/L toluene and 100 dB of white noise in a chamber specifically designed for the purpose over two consecutive weeks. Serum levels of TNF-α and IL-1β were measured with the enzyme-linked immunosorbent assay (ELISA), whereas Bax and Bcl-2 expressions in tissues were determined with real-time polymerase chain reaction (PCR). Noise and toluene changed TNF-α and IL-1β serum levels on different days following the end of exposure and significantly increased the Bax/Bcl-2 ratio in the lung and spleen. In addition, they induced different pathological changes in the heart, lung, spleen, and stomach tissues. This study has confirmed that exposure to noise and toluene can induce a range of toxicopathological changes, probably by inducing inflammatory pathways and apoptosis, but their combined effects look weaker than those of its components, although histopathological findings suggest the opposite.

Published
2022-03-25
How to Cite
1.
Abouee-Mehrizi A, Rasoulzadeh Y, Kazemi T, Mehdipour A, Mesgari-Abbasi M. Toxicopathological changes induced by combined exposure to noise and toluene in New Zealand White rabbits. Arh Hig Rada Toksikol [Internet]. 2022Mar.25 [cited 2022Jun.30];73(1). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/1451
Section
Original article