3-nitrotyrosine levels in dichlorvos-induced neurotoxicity

  • Dilek Guvenç Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun
  • Abdurrahman Aksoy Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun
  • Yavuz Kursad Das Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun
  • Enes Atmaca Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun
  • Oguzhan Yavuz Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun
DOI: https://doi.org/10.2478/10004-1254-65-2014-2416
Keywords: brain, HPLC, nitro-oxidative stress, pesticides, rats

Abstract

The aim of this study was to evaluate dichlorvos toxicity in terms of nitro-oxidative stress by determining 3-nitrotyrosine (3-NT) levels in the fore, mid, and hindbrain regions in acutely exposed rats. Male Sprague-Dawley rats were randomly allocated to three groups of eight. Group 1 was administered a single intraperitoneal dichlorvos dose of 1.8 mg kg-1 (0.1xLD50) and group 2 a dose of 9 mg kg-1 (0.5xLD50). The control group received 0.5 mL saline solution via the same route. 3-NT and tyrosine (TYR) levels were measured using high performance liquid chromatography with a photodiode array detector (HPLC-PDA) and expressed as a ratio of 3-NT to TYR. The 3-NT/1000 TYR ratios increased significantly in the fore-, mid- and hindbrains of the exposed groups compared to control (p<0.01). In the forebrain, the increase was also significant between the treated groups. Our study has confirmed that acute exposure to dichlorvos leads to nitro-oxidative stress in the brain and that 3-NT may play a role in the mechanism of dichlorvos neurotoxicity.

Published
2014-03-15
How to Cite
1.
Guvenç D, Aksoy A, Das YK, Atmaca E, Yavuz O. 3-nitrotyrosine levels in dichlorvos-induced neurotoxicity. Arh Hig Rada Toksikol [Internet]. 2014Mar.15 [cited 2024Apr.20];65(1). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/149
Issue
Vol 65 No 1 (2014)
Section
Original article