Absence of mutations in the human interferon alpha-2b gene in workers chronically exposed to ionising radiation

  • Dauren M. Botbayev Аitkhozhin Institute of Moleculаr Biology аnd Biochemistry KS MES, Аlmаty
  • Gloria Ravegnini Department of Pharmacy and Biotechnology, University of Bologna, Bologna
  • Giulia Sammarini Department of Pharmacy and Biotechnology, University of Bologna, Bologna
  • PK Kazymbet Institute of Radiobiology and Radiation Protection, Medical University, Astana
  • Elisabetta Cilli Laboratories of Physical Anthropology and Ancient DNA, Department of Cultural Heritage, University of Bologna, Ravenna Campus
  • Patrizia Serventi Laboratories of Physical Anthropology and Ancient DNA, Department of Cultural Heritage, University of Bologna, Ravenna Campus
  • AK Khanseitova Аitkhozhin Institute of Moleculаr Biology аnd Biochemistry KS MES, Аlmаty
  • B Alzhanuly Аitkhozhin Institute of Moleculаr Biology аnd Biochemistry KS MES, Аlmаty
  • AM Belkozhaev Аitkhozhin Institute of Moleculаr Biology аnd Biochemistry KS MES, Аlmаty
  • NA Aitkhozina Аitkhozhin Institute of Moleculаr Biology аnd Biochemistry KS MES, Аlmаty
  • M Bakhtin Institute of Radiobiology and Radiation Protection, Medical University, Astana
  • Patrizia Hrelia Department of Pharmacy and Biotechnology, University of Bologna, Bologna
  • Sabrina Angelini Universita di BolognaFacoltà di Farmacia, Dipartimento di Farmacologia
Keywords: hIFNα-2b mutations, genotoxicity, radiology workers, uranium miners

Abstract

Individuals chronically exposed to low-level ionising radiation (IR) run the risk of harmful and long-term adverse health effects, including gene mutations and cancer development. The search for reliable biomarkers of IR exposure in human population is still of great interest, as they may have a great implementation potential for the surveillance of occupationally exposed individuals. In this context, and considering previous literature, this study aimed to identify mutations in the human interferon alpha-2b (hIFN?-2b) as a potential biomarker of occupational chronic low-dose IR exposure linking low-IR exposure to the effects on haematopoiesis and reduced immunity. The analysis was performed in the genomic DNA of 51 uranium miners and 38 controls from Kazakhstan, and in 21 medical radiology workers and 21 controls from Italy. hIFN?-2b gene mutations were analysed with the real-time polymerase chain reaction (PCR) or Sanger sequencing. However, none of the investigated workers had the hIFN?-2b mutation. This finding highlights the need for further research to identify biomarkers for early detection of health effects associated with chronic low-dose IR exposure.

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Published
2019-04-30
How to Cite
1.
Botbayev DM, Ravegnini G, Sammarini G, Kazymbet P, Cilli E, Serventi P, Khanseitova A, Alzhanuly B, Belkozhaev A, Aitkhozina N, Bakhtin M, Hrelia P, Angelini S. Absence of mutations in the human interferon alpha-2b gene in workers chronically exposed to ionising radiation. Arh Hig Rada Toksikol [Internet]. 2019Apr.30 [cited 2019Oct.23];70(2). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/1034
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Original article

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