A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44

Authors

Katsuhiko Warita Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
Tomoko Mitsuhashi Department of Pathology, University of Pittsburgh, School of Medicine
Nobuhiko Hoshi Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University
Ken-ichi Ohta Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
Shingo Suzuki Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
Yoshiki Takeuchi Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
Takanori Miki Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University

DOI:

https://doi.org/10.2478/10004-1254-65-2014-2494

Keywords:

Caspase 3, developmental toxicity, foetal hypothalamus, gene expression, nerve growth factor (Ngf), transformation related protein 73 (Trp73)

Abstract

We investigated the toxicity of bisphenol A (BPA) by determining the gene expression of nerve growth factor (Ngf) in the embryonic mouse cell line mHypoE-N44 derived from the hypothalamus exposed to BPA dose range between 0.02 and 200 µmol L-1 for 3 h. Ngf mRNA levels decreased in a dose-dependent manner, with significant reductions observed in the 2 to 50 µmol L-1 BPA treatment groups compared to controls. However, at 100 to 200 µmol L-1 the Ngf mRNA gradually increased and was significantly higher than control, while the expression of the apoptosis-related genes Caspase 3 and transformation-related protein 73 decreased significantly. These results suggest that in an embryonic hypothalamic cell line the higher doses of BPA induce a unique pattern of Ngf gene expression and that BPA has the potential to suppress apoptosis essential for early-stage brain development.

Author Biographies

Katsuhiko Warita, Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University

Assistant professor
Tomoko Mitsuhashi, Department of Pathology, University of Pittsburgh, School of Medicine

Postdoctoral research fellow
Nobuhiko Hoshi, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University

Professor
Ken-ichi Ohta, Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University

Assistant professor
Shingo Suzuki, Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University

Assistant professor
Yoshiki Takeuchi, Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University

Professor
Takanori Miki, Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University

Associate professor

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Published

08.08.2014

Issue

Vol. 65 No. 3 (2014)

Section

Original article

How to Cite

Warita K, Mitsuhashi T, Hoshi N, Ohta K- ichi, Suzuki S, Takeuchi Y, et al. A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44. Arh Hig Rada Toksikol [Internet]. 2014 Aug. 8 [cited 2025 Apr. 22];65(3). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/134