A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44
Keywords:
Caspase 3, developmental toxicity, foetal hypothalamus, gene expression, nerve growth factor (Ngf), transformation related protein 73 (Trp73)
Abstract
We investigated the toxicity of bisphenol A (BPA) by determining the gene expression of nerve growth factor (Ngf) in the embryonic mouse cell line mHypoE-N44 derived from the hypothalamus exposed to BPA dose range between 0.02 and 200 µmol L-1 for 3 h. Ngf mRNA levels decreased in a dose-dependent manner, with significant reductions observed in the 2 to 50 µmol L-1 BPA treatment groups compared to controls. However, at 100 to 200 µmol L-1 the Ngf mRNA gradually increased and was significantly higher than control, while the expression of the apoptosis-related genes Caspase 3 and transformation-related protein 73 decreased significantly. These results suggest that in an embryonic hypothalamic cell line the higher doses of BPA induce a unique pattern of Ngf gene expression and that BPA has the potential to suppress apoptosis essential for early-stage brain development.
Published
2014-08-08
How to Cite
1.
Warita K, Mitsuhashi T, Hoshi N, Ohta K- ichi, Suzuki S, Takeuchi Y, Miki T. A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44. Arh Hig Rada Toksikol [Internet]. 2014Aug.8 [cited 2024Apr.19];65(3). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/134
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