A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44

Authors

  • Katsuhiko Warita Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
  • Tomoko Mitsuhashi Department of Pathology, University of Pittsburgh, School of Medicine
  • Nobuhiko Hoshi Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University
  • Ken-ichi Ohta Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
  • Shingo Suzuki Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
  • Yoshiki Takeuchi Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
  • Takanori Miki Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University

DOI:

https://doi.org/10.2478/10004-1254-65-2014-2494

Keywords:

Caspase 3, developmental toxicity, foetal hypothalamus, gene expression, nerve growth factor (Ngf), transformation related protein 73 (Trp73)

Abstract

We investigated the toxicity of bisphenol A (BPA) by determining the gene expression of nerve growth factor (Ngf) in the embryonic mouse cell line mHypoE-N44 derived from the hypothalamus exposed to BPA dose range between 0.02 and 200 µmol L-1 for 3 h. Ngf mRNA levels decreased in a dose-dependent manner, with significant reductions observed in the 2 to 50 µmol L-1 BPA treatment groups compared to controls. However, at 100 to 200 µmol L-1 the Ngf mRNA gradually increased and was significantly higher than control, while the expression of the apoptosis-related genes Caspase 3 and transformation-related protein 73 decreased significantly. These results suggest that in an embryonic hypothalamic cell line the higher doses of BPA induce a unique pattern of Ngf gene expression and that BPA has the potential to suppress apoptosis essential for early-stage brain development.

Author Biographies

  • Katsuhiko Warita, Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
    Assistant professor
  • Tomoko Mitsuhashi, Department of Pathology, University of Pittsburgh, School of Medicine

    Postdoctoral research fellow

  • Nobuhiko Hoshi, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University
    Professor
  • Ken-ichi Ohta, Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
    Assistant professor
  • Shingo Suzuki, Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
    Assistant professor
  • Yoshiki Takeuchi, Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
    Professor
  • Takanori Miki, Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University
    Associate professor

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Published

08.08.2014

Issue

Vol. 65 No. 3 (2014)

Section

Original article

How to Cite

1.
A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44. Arh Hig Rada Toksikol [Internet]. 2014 Aug. 8 [cited 2025 Jan. 22];65(3). Available from: https://arhiv.imi.hr/index.php/arhiv/article/view/134