Archives of Industrial Hygiene and Toxicology 2022-09-29T13:04:46+02:00 Arhiv za higijenu rada i toksikologiju Open Journal Systems <div class="WordSection1"> <p><strong><em>Archives of Industrial Hygiene and Toxicology</em></strong> (Arh Hig Rada Toksikol) is an internationally peer-reviewed biomedical scientific quarterly that publishes contributions relevant to all aspects of environmental and occupational health and toxicology.</p> <p>Indexed in <strong>SCI Expanded</strong>,<strong> Medline</strong>/<strong>PubMed</strong>,<strong> Scopus</strong>, Animal Science Database, Biological Sciences (CSA), BIOSIS Previews, GreenFile, INIS, Pollution Abstracts, Veterinary Science Database, Water Resources Abstracts, EBSCO Academic Search Complete, TEMA, TOXLINE, AGRIS, Food Science and Technology Abstracts – FSTA, and Ergonomic Abstracts.</p> <p><em>Archives</em> is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE).</p> <p>Impact Factor:<strong> 2.078</strong></p> <p>5-year Impact Factor:<strong><strong> 2.804</strong></strong></p> </div> Medical devices as a source of phthalate exposure: a review of current knowledge and alternative solutions 2022-09-29T12:59:00+02:00 Antonela Šimunović Siniša Tomić Krunoslav Kranjčec <p>Phthalates are a group of phthalic acid esters used as plasticisers in a large number of products to improve their flexibility, softness, and extensibility. Their wide use in medical devices, however, raises a lot of concern, as they can enter the organism and have toxic effects on human liver, thyroid, kidneys, lungs, reproductive, endocrine, nervous, and respiratory system and are associated with asthma, obesity, autism, and diabetes. The aim of this review is to summarise current knowledge about phthalate migration from medical devices during different medical procedures and possible impact on patient health. It also looks at alternative plasticisers with supposedly lower migration rates and safer profile. Not enough is known about which and how many phthalates make part of medical devices or about the health impacts of alternative plasticisers or their migration rates.</p> 2022-09-15T07:58:53+02:00 Copyright (c) 2022 Antonela Šimunović, Siniša Tomić, Krunoslav Kranjčec Prenatal and perinatal phthalate exposure is associated with sex-dependent changes in hippocampal miR-15b-5p and miR-34a-5p expression and changes in testicular morphology in rat offspring 2022-09-29T12:59:02+02:00 Peter Štefánik Jaroslav Michalec Martina Morová Lucia Olexová Lucia Kršková <p>MicroRNAs are a large group of non-coding nucleic acids, usually 20–22&nbsp;nt long, which bind to regulatory sections of messenger RNA (mRNA) and inhibit gene expression. However, genome activity is also regulated by hormones. Endocrine disruptors such as those from the phthalate group imitate or block these hormonal effects, and our previous study showed a long-lasting decrease in plasma testosterone levels in rat offspring exposed to a mixture of three phthalates <em>in utero</em> and postnatally. These effects were also observed at the behavioural level. To shed more light on these findings, in this new study we compared testicular tissue morphology between control and phthalate-treated males and investigated possible persistent changes and sex differences in the expression of two hippocampal microRNAs – miR-15b-5p and miR-34a-5p – participating in the transcription of steroidogenic genes. Histologically observed changes in testicular tissue morphology of phthalate-exposed males compared to control support testosterone drop observed in the previous study. At the microRNA level, we observed more significant changes in phthalate-treated females than in males. However, we are unable to relate these effects to the previously observed behavioural changes.</p> 2022-09-21T11:39:15+02:00 Copyright (c) 2022 Peter Štefánik, Jaroslav Michalec, Martina Morová, Lucia Olexová, Lucia Kršková Whey protein protects liver mitochondrial function against oxidative stress in rats exposed to acrolein 2022-09-29T12:59:03+02:00 Birsen Aydın Ali Oğuz Vedat Şekeroğlu Zülal Atlı Şekeroğlu <p>Acrolein (AC) is one of the most toxic environmental pollutants, often associated with incomplete combustion of petrol, wood, and plastic, oil frying, and tobacco smoking, that causes oxidative damage to DNA and mitochondria. Considering that little is known about the protective effects of whey protein (WP) against AC-induced liver toxicity, the aim of our study was to learn more about them in respect to liver mitochondrial oxidative stress, respiratory enzymes, Krebs cycle enzymes, and adenosine triphosphate (ATP). To do that, we treated Sprague Dawley rats with daily doses of AC alone (5&nbsp;mg/kg bw in 0.9&nbsp;% NaCl solution), WP alone (200&nbsp;mg/kg bw, in 0.9&nbsp;% NaCl solution), or their combination by oral gavage for six days a week over 30 days. As expected, the AC group showed a drop in glutathione levels and antioxidant, transport chain, and tricarboxylic acid cycle enzyme activities and a significant rise in mitochondrial lipid peroxidation and protein carbonyl levels. Co-treatment with WP mitigated oxidative stress and improved enzyme activities. Judging by the measured parameters, WP reduced AC toxicity by improving bioenergetic mechanisms and eliminating oxidative stress.</p> 2022-09-15T07:58:26+02:00 Copyright (c) 2022 Birsen Aydın, Ali Oğuz, Vedat Şekeroğlu, Zülal Atlı Şekeroğlu Redox and biometal status in Wistar rats after subacute exposure to fluoride and selenium counter-effects 2022-09-29T12:59:05+02:00 Jelena Radovanović Biljana Antonijević Katarina Baralić Marijana Ćurčić Danijela Đukić-Ćosić Zorica Bulat Dragana Javorac Aleksandra Buha Đorđević Jelena Kotur-Stevuljević Emina Sudar-Milovanović Evica Antonijević Miljaković Miloš Beloica Zoran Mandinić <p>This study aimed to investigate the effect of 150&nbsp;mg/L sodium fluoride (NaF) on redox status parameters and essential metals [copper (Cu), iron (Fe), and zinc (Zn)] in the blood, liver, kidney, brain, and spleen of Wistar rats and to determine the protective potential of selenium (Se) against fluoride (F<sup>-</sup>) toxicity. Male Wistar rats were randomly distributed in groups of five (n=5) receiving tap water (control) or water with NaF 150&nbsp;mg/L, NaF 150&nbsp;mg/L + Se 1.5&nbsp;mg/L, and Se 1.5&nbsp;mg/L solutions <em>ad libitum </em>for 28 days. Fluorides caused an imbalance in the redox and biometal (Cu, Fe, and Zn) status, leading to high superoxide anion (O<sub>2</sub><sup>.-</sup>) and malondialdehyde (MDA) levels in the blood and brain and a drop in superoxide dismutase (SOD1) activity in the liver and its increase in the brain and kidneys. Se given with NaF improved MDA, SOD1, and O<sub>2</sub><sup>.-</sup> in the blood, brain, and kidneys, while alone it decreased SH group levels in the liver and kidney. Biometals both reduced and increased F<sup>-</sup> toxicity. Further research is needed before Se should be considered as a promising strategy for mitigating F<sup>-</sup> toxicity.</p> 2022-09-15T07:57:58+02:00 Copyright (c) 2022 Jelena Radovanović, Biljana Antonijević, Katarina Baralić, Marijana Ćurčić, Danijela Đukić-Ćosić, Zorica Bulat, Dragana Javorac, Aleksandra Buha Đorđević, Jelena Kotur-Stevuljević, Emina Sudar-Milovanović, Evica Antonijević Miljaković, Miloš Beloica, Zoran Mandinić The effect of low doses of chlorpyrifos on blood and bone marrow cells in Wistar rats 2022-09-29T12:59:07+02:00 Vilena Kašuba Vedran Micek Mirta Milić Davor Želježić Anja Katić <p>The aim of this study was to investigate the genotoxic potential of low doses of chlorpyrifos (CPF) on blood and bone marrow cells in adult male Wistar rats. CPF was administered by oral gavage at daily doses of 0.010, 0.015, and 0.160&nbsp;mg/kg of body weight (bw) for 28 consecutive days. Positive control (PC) was administered 300&nbsp;mg/kg bw/day of ethyl methane sulphonate (EMS) for the final three days of the experiment. Toxic outcomes of exposure were determined with the <em>in vivo</em> micronucleus (MN) assay and alkaline comet assay. The 28-day exposure to the 0.015&nbsp;mg/kg CPF dose, which was three times higher than the current value of acute reference dose (ARfD), reduced body weight gain in rats the most. The <em>in vivo</em> MN assay showed significant differences in number of reticulocytes per 1000 erythrocytes between PC and negative control (NC) and between all control groups and the groups exposed to 0.015 and 0.160&nbsp;mg/kg bw/day of CPF. The number of micronucleated polychromatic erythrocytes per 2000 erythrocytes was significantly higher in the PC than the NC group or group exposed to 0.015&nbsp;mg/kg bw/day of CPF. CPF treatment did not significantly increase primary DNA damage in bone marrow cells compared to the NC group. However, the damage in bone marrow cells of CPF-exposed rats was much higher than the one recorded in leukocytes, established in the previous research. Both assays proved to be successful for the assessment of CPF-induced genome instability in Wistar rats. However, the exact mechanisms of damage have to be further investigated and confirmed by other, more sensitive methods.</p> 2022-09-29T12:34:04+02:00 Copyright (c) 2022 Vilena Kašuba, Vedran Micek, Mirta Milić, Davor Želježić, Anja Katić Occupational burnout among teachers: is it seasonal? 2022-09-29T12:59:08+02:00 Nada Marić Stefan Mandić-Rajčević Nataša Maksimović Petar Bulat <p>The aim of this cross-sectional study was to compare occupational burnout in two groups of teachers from the district of Bijeljina (Bosnia and Herzegovina) measured with the Serbian version of the Maslach Burnout Inventory survey for workers in human services (MBI-HSS) at the beginning (group 1) and the end of the school year 2018/2019 (group 2) to see if there are seasonal differences. The questionnaire also included standard sociodemographic data and job description (primary and/or secondary school position, length of service, and overtime work). The prevalence of emotional exhaustion and depersonalisation was low in both groups. However, emotional exhaustion and depersonalisation scores significantly shifted to higher values between the beginning and the end of the school year. We also found a statistically significant association between emotional exhaustion and overtime and between depersonalisation and work in a secondary school (p&lt;0.05). These findings invite further research of occupational burnout seasonality in schoolteachers, preferably by following up cohorts which would be controlled for sociodemographic and work-related variables.</p> 2022-09-29T12:10:02+02:00 Copyright (c) 2022 Nada Marić, Stefan Mandić-Rajčević, Nataša Maksimović, Petar Bulat Comments on “Wi-Fi technology and human health impact: a brief review of current knowledge” published in the June 2022 issue of Archives 2022-09-29T13:04:46+02:00 Enrique Arribas Isabel Escobar Antonio Martinez-Plaza Raquel Ramirez-Vazquez 2022-09-29T13:04:01+02:00 Copyright (c) 2022 Enrique Arribas, Isabel Escobar, Antonio Martinez-Plaza, Raquel Ramirez-Vazquez