TY - JOUR AU - Teuta Murati AU - Marina Miletić AU - Josipa Kolarić AU - Vanja Lovrić AU - Danijela Bursać Kovačević AU - Predrag Putnik AU - Irena Landeka Jurčević AU - Domagoj Đikić AU - Verica Dragović-Uzelac AU - Ivana Kmetič PY - 2019/11/25 Y2 - 2024/03/28 TI - Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells JF - Archives of Industrial Hygiene and Toxicology JA - Arh Hig Rada Toksikol VL - 70 IS - 4 SE - Original article DO - 10.2478/aiht-2019-70-3322 UR - https://arhiv.imi.hr/index.php/arhiv/article/view/1169 AB - Prunus spinosa L. (blackthorn) is used in traditional medicine as a remedy for various diseases. To establish its anticancer properties, we exposed human liver cancer cells (Hep G2) to a range of blackthorn flower extract concentrations (10–200 µg/mL) and determined cytotoxic activity with the neutral red and kenacid blue methods after 24, 48, and 72 h of incubation. Statistically significant inhibitory effects on Hep G2 cellular proliferation were observed at concentrations above 50 µg/mL (p<0.001–0.05). Cell viability was lower when determined with neutral red than kenacid blue method. In addition, we evaluated antioxidant/prooxidant effects of the blackthorn flower extract by measuring reactive oxygen species (ROS), and the results confirmed its prooxidant behaviour within the applied concentration range. Flow cytometry determined primarily necrotic and apoptotic cell death, which provides additional evidence of its cytotoxic effect on liver carcinoma. ER -